What is Phosphate Buffered Saline (PBS) used for?

Phosphate Buffered Saline (PBS) is a versatile buffer solution commonly used in biological and biochemical research. It provides a stable pH environment, typically 7.4, which is crucial for processes like cell culture, protein purification, and enzyme activity assays. By maintaining osmotic balance and providing essential ions, PBS helps maintain cell viability during experiments. For instance, it's commonly used to dilute samples or as a wash buffer in staining protocols.

What does 'ultra-low endotoxin' mean in this PBS product?

The term 'ultra-low endotoxin' indicates that the PBS buffer has been processed to minimize the presence of bacterial endotoxins, which can interfere with experimental results, particularly in sensitive assays. This is essential for applications like cell culture and molecular biology experiments, where even trace amounts of endotoxins can affect cell behavior or assay accuracy. By using ultra-low endotoxin PBS, researchers can ensure that their experiments yield reliable and reproducible results.

How is the filtration process done for this PBS?

This PBS is filtered using a 0.1um filtration system, which effectively removes bacteria and larger particles, ensuring that the solution is sterile and suitable for laboratory use. This stringent filtration process is crucial for applications where contamination can lead to erroneous findings. Researchers often use this type of PBS for preparing cell suspensions or during washing steps in immunohistochemistry to ensure that their samples remain uncontaminated and of high quality.

Why is the pH of 7.4 important?

A pH of 7.4 is optimal for many biological applications because it closely matches the physiological conditions of human and animal tissues. This allows for more realistic environments when growing cells or conducting assays, which is essential for gathering relevant and applicable data. For example, using PBS at this pH for cell culture helps in maintaining proper cellular function, promoting growth, and achieving accurate experimental outcomes.

How do I dilute this 1× concentrate PBS for my experiments?

To use the 1× concentrate PBS effectively, it's typically diluted with distilled water to reach the desired application concentration. For example, if you need a standard working solution, you can take the designated volume of the 1× concentrate and dilute it accordingly. This makes it suitable for a range of applications, from cell maintenance to washing steps in immunoassays, ensuring that your experimental conditions are met accurately.

What is the significance of using a 500ml size for PBS?

The 500ml packaging size is ideal for labs that require a significant volume of PBS while ensuring that it remains sterile and easy to handle. This size allows researchers to use it for multiple experiments, stock solutions, or routine applications without frequent replacements. It’s practical for laboratories conducting high-throughput assays or daily cell culture, reducing the risk of contamination from repeated openings of smaller containers.

Can this PBS be used for tissue culture applications?

Yes, this phosphate buffered saline is suitable for tissue culture applications. Its pH-balanced, sterile formulation provides an optimal environment for maintaining cellular integrity during experimental procedures. It's often utilized for trypsinizing and rinsing cultured cells, which is vital to ensure that cells remain healthy and viable for experimentation. By using this PBS, researchers can enhance the reliability of their cell-based assays.

Are there any specific safety precautions I need to take when using this PBS?

When using Phosphate Buffered Saline, it is essential to handle it under standard laboratory safety protocols. Wearing gloves, goggles, and lab coats is recommended to prevent any accidental contact. Additionally, ensure to work in a sterile environment to avoid contamination. These precautions are vital, especially in sensitive experiments where accuracy is paramount, such as those involving cell lines or other biological materials.

What is the composition of this PBS buffer?

This PBS buffer typically consists of sodium chloride, potassium chloride, sodium phosphate, and potassium phosphate dissolved in distilled water, with a pH of 7.4. The precise ionic composition helps in mimicking physiological conditions, which is crucial for various laboratory experiments. Understanding the composition aids researchers in choosing the right buffer for their specific applications, ensuring that experimental conditions are optimal for cell growth or biochemical assays.

Where can I buy Phosphate Buffered Saline 1× Ultra-Low Endotoxin?

You can purchase Phosphate Buffered Saline 1× Ultra-Low Endotoxin from Ubuy in Pakistan. Ubuy offers a wide selection of laboratory products, including PBS, ensuring that you receive high-quality solutions for your experimental needs. Shopping at Ubuy allows you to access efficient ordering and product descriptions that cater to laboratory professionals and researchers alike.

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Is PBS Buffer Ultra-Low Endotoxin, Phosphate Buffered Available to Shop Online in Pakistan?

Yes, at Ubuy Pakistan this product is available for you to shop at a reasonable price. The PBS Buffer Ultra-Low Endotoxin, Phosphate Buffered is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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PBS Buffer Ultra-Low Endotoxin, Phosphate Buffered Saline, 500mL | Endotoxin 0.05 EU/mL, Ready-to-Use Reagent, 0.1m Filtered, Laboratory Cell Culture Workflows

Our Ultra-Low Endotoxin PBS Buffer is specially formulated to minimize endotoxin levels, ensuring optimal conditions for sensitive cell-based research.

CryoKing

Item #: 241493803

PBS Buffer Ultra-Low Endotoxin, Phosphate Buffered Saline, 500mL | Endotoxin 0.05 EU/mL, Ready-to-Use Reagent, 0.1m Filtered, Laboratory Cell Culture

Item #: 241493803

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Ultra-Low Endotoxin

Specifically formulated with an endotoxin level of 0.05 EU/mL, ensuring minimal contamination for sensitive cell culture applications, enhancing the reliability of experimental results.

Ready-to-Use

Conveniently packaged in a 500mL volume, eliminating the need for preparation. This user-friendly reagent saves valuable lab time while maintaining high consistency in experiments.

0.1m Filtered

Each batch is rigorously filtered to 0.1 microns, ensuring the removal of particulates and contaminants. This filtering process guarantees optimal sterility for critical laboratory workflows.

Product Details

Brand Name	CryoKing
Unit Count	1 Count
Manufacturer	BIOLOGIX
Included Components	PBS Buffer
Part Number	PBS-501-1
Brand	CryoKing
Item Weight	10 lbs (4.54 kg)

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Cell Culture Labs
Ideal for laboratories focusing on cell culture requiring low endotoxin levels for reliable cell growth and experimentation.
Pharmaceutical Research
Essential for pharmaceutical research where endotoxin contamination could affect drug efficacy and safety assessments.
Diagnostic Testing
Beneficial for diagnostic labs conducting assays that demand high purity and low endotoxin levels to ensure accurate results.

Non-Laboratory Use
Not suitable for casual or non-scientific applications where strict contamination controls are unnecessary or impractical.

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PBS Buffer Ultra-Low Endotoxin, Phosphate Buffered Saline, 500mL | Endotoxin 0.05 EU/mL, Ready-to-Use Reagent, 0.1m Filtered, Laboratory Cell Culture Workflows

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Question: How to Shop PBS Buffer Ultra-Low Endotoxin, Phosphate Buffered Online From Ubuy?

Answer: It’s easy to shop PBS Buffer Ultra-Low Endotoxin, Phosphate Buffered online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is PBS Buffer Ultra-Low Endotoxin, Phosphate Buffered Available to Shop Online in Pakistan?

Answer: Yes, at Ubuy Pakistan this product is available for you to shop at a reasonable price. The PBS Buffer Ultra-Low Endotoxin, Phosphate Buffered is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Features & Benefits

Ultra-Low Endotoxin Level (≤0.05 EU/mL) for enhanced cell health.
Versatile applications including tissue rinsing and reagent preparation.
Strict quality control for consistent purity and reliability.
pH-Stable at 7.4 for balanced cell culture environments.
Carefully produced to minimize endotoxin content, supporting accurate research outcomes.
Ideal for cell culture, immunoassays, and laboratory research.

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PBS Buffer Ultra-Low Endotoxin, Phosphate Buffered Saline, 500mL | Endotoxin 0.05 EU/mL, Ready-to-Use Reagent, 0.1m Filtered, Laboratory Cell Culture

PKR 9014

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