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50x TAE Buffer 4 L
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50x TAE Buffer 4 L
Recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA.
50x TAE Buffer 4 L
Item #: 116962390

50x TAE Buffer 4 L

Item #: 116962390

PKR 59161

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Recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA.
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What Stands Out

High Concentration
This 50x TAE buffer offers a concentrated formula, ensuring optimal performance for nucleic acid electrophoresis, reducing the need for frequent replenishment and saving on resources.
Large Volume
With a generous 4-liter volume, this buffer is perfect for extensive laboratory usage, catering to high-throughput research needs and minimizing interruptions during experiments.
Quality Assurance
Produced under strict quality control, this TAE buffer guarantees consistent and reliable results, providing confidence to researchers and professionals in their molecular biology applications.

Product Details

Shop 50x TAE Buffer 4 L online at a best price in Pakistan. B0DJQ277BC
Item Weight8.8 lbs (3.99 kg)

Who Should Buy?

Suitable For
  • Molecular Biologists

    Essential for gel electrophoresis, providing the required ionic strength and pH stability for DNA and RNA analysis.

  • Educational Institutions

    Useful for teaching laboratories, enabling hands-on experiments for students in molecular biology and genetics courses.

  • Research Laboratories

    Perfect for continuous usage in experiments requiring buffer solutions, reducing the need for frequent preparation.

Not Suitable For
  • Casual Users

    Not suitable for individuals with no background in molecular biology or those not performing laboratory experiments.

  • Small-Scale Experiments

    A 4 L quantity may be excessive for minimal experiments, leading to waste and unnecessary costs.

  • Field Applications

    Not ideal for outdoor or non-lab settings where stable storage and handling conditions are challenging.

Product Description

50x TAE Buffer 4 L

Have any Query? Chat with us

Customer Questions & Answers

  • Question: What is 50x TAE Buffer 4 L?

    Answer: 50x TAE Buffer 4 L is a laboratory reagent used in molecular biology for running agarose gel electrophoresis. It acts as a buffering agent, maintaining a stable pH environment during the separation of nucleic acids.
    Category: overview
    Confidence: high
  • Question: How do I prepare 1x TAE Buffer from 50x TAE Buffer?

    Answer: To prepare 1x TAE Buffer from 50x, dilute the 50x solution by mixing 1 part of 50x TAE Buffer with 49 parts of distilled water. This creates a final solution suitable for use in electrophoresis.
    Category: setup
    Confidence: high
  • Question: What are the components of 50x TAE Buffer?

    Answer: 50x TAE Buffer typically contains Tris base, acetic acid, and EDTA, which help to maintain the buffer's pH and chelate divalent metal ions. These components are critical for effective DNA separation in gel electrophoresis.
    Category: specs
    Confidence: high
  • Question: Can I store 50x TAE Buffer, and if so, how?

    Answer: Yes, store 50x TAE Buffer at room temperature or in a refrigerator. Ensure the container is tightly sealed to prevent contamination, and avoid direct sunlight to maintain its efficacy.
    Category: maintenance
    Confidence: high
  • Question: Is 50x TAE Buffer safe to use?

    Answer: 50x TAE Buffer is generally safe for laboratory use, but standard safety precautions should be followed, such as wearing gloves and goggles. It is essential to handle all reagents properly to avoid any accidents.
    Category: safety
    Confidence: high
  • Question: What is the shelf life of 50x TAE Buffer?

    Answer: The shelf life of 50x TAE Buffer can vary by manufacturer, but it is usually stable for at least 2 years when stored correctly. Check the product label for specific expiration information.
    Category: other
    Confidence: medium
  • Question: What equipment is needed to use 50x TAE Buffer?

    Answer: To use 50x TAE Buffer, you will need an electrophoresis apparatus, agarose gel, power supply, and pipettes or other methods for precise dilution and handling of the buffer solution.
    Category: setup
    Confidence: high
  • Question: Can 50x TAE Buffer be reused?

    Answer: Reusing TAE Buffer is not recommended as it can become contaminated or lose effectiveness. Fresh buffer solutions should be prepared for each electrophoresis run for optimal results.
    Category: usage
    Confidence: high
  • Question: What is the pH of 1x TAE Buffer?

    Answer: The pH of 1x TAE Buffer is typically around 8.0. This pH is suitable for most nucleic acid applications, including DNA fragment analysis.
    Category: specs
    Confidence: high
  • Question: How does 50x TAE Buffer compare to other buffers?

    Answer: 50x TAE Buffer provides better resolution for larger DNA fragments compared to TBE Buffer. However, TBE may offer higher buffering capacity. The choice depends on the specific requirements of your experiment.
    Category: other
    Confidence: medium
  • Question: Is 50x TAE Buffer compatible with all DNA gels?

    Answer: Yes, 50x TAE Buffer is compatible with a variety of DNA agarose gels. It is commonly used in techniques requiring DNA separation, such as gel electrophoresis.
    Category: compatibility
    Confidence: high
  • Question: What should I do if I notice precipitation in the buffer?

    Answer: If you notice precipitation in 50x TAE Buffer, gently warm it to dissolve any crystals. Ensure the buffer is mixed thoroughly before use to maintain its effectiveness.
    Category: troubleshooting
    Confidence: medium
  • Question: Can 50x TAE Buffer be used for RNA electrophoresis?

    Answer: While 50x TAE Buffer is primarily designed for DNA, it can also be used for RNA electrophoresis, although TBE Buffer is often preferred for RNA due to its lower promoting of RNA degradation.
    Category: compatibility
    Confidence: medium
  • Question: What are the environmental disposal guidelines for 50x TAE Buffer?

    Answer: 50x TAE Buffer should be disposed of according to local environmental regulations. It is generally safe to wash small quantities down the drain with plenty of water, but check specific disposal guidelines to ensure compliance.
    Category: other
    Confidence: medium

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PKR 59161

Order now and get it around Thursday, July 09

QTY:

Secured transactionsecured transaction

Ubuy works hard to protect your security and privacy. Our advanced payment security system ensures confidentiality by encrypting your information during transmission using AES (Advanced Encryption Standards) and SSL (Secure Socket Layer) protocols. Your payment details are 100% secure as we do not share your payment details with third party sellers.

Our Top Logistics Partners
  • fedex
  • dhl
Fastest cross-border delivery

Features & Benefits

  • Ideal for electrophoresis of nucleic acids in agarose and polyacrylamide gels.
  • Can be used as both a running buffer and a gel preparation buffer.
  • Filtered through a 0.22 µm membrane for enhanced purity.
  • Exceptional for resolving RNA and DNA fragments larger than 1500 bp.
  • Formulated with Tris, Acetate, and EDTA at pH 8.0.
  • Perfect for genomic DNA and large supercoiled DNA applications.