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Diamine Oxidase Assay Kit (QFDO-100)
PKR 219694
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Fast, sensitive, and convenient DAO assay for high-throughput testing of histamine intolerance.
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What Stands Out
Product Details
- For quantitative determination of diamine o x idase in biological samples (e
- g
- serum, plasma etc) Linear detection range 0
- 5 to 6 U/L
- Convenient room temperature assay
Product Technical Details
| Product Dimensions | 4 x 2 x 5.5 inches |
| Item Weight | 1 pounds |
| Manufacturer | BioAssay |
| Item model number | QFDO-100 |
| Is Discontinued By Manufacturer | No |
What's in the box
Kit
Who Should Buy?
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Researchers in Biomedicine
Ideal for scientists studying histamine metabolism and related health conditions through the measurement of diamine oxidase activity.
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Clinics and Diagnostic Labs
Useful for healthcare providers needing to assess patients for conditions related to high histamine levels and intolerance.
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Food Safety Analysts
Beneficial for those checking histamine levels in food products to ensure compliance with safety standards and regulations.
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Casual Users
Not suitable for individuals seeking a general health measure, as it requires technical expertise for accurate results.
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Non-Scientist Hobbyists
May not be the best fit for enthusiasts without a background in biochemistry or assay handling for effective use.
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General Health Monitoring
Not intended for routine health assessments, focusing specifically on diamine oxidase activity rather than comprehensive health indicators.
Product Description
Customer Questions & Answers
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Question:
What is the Diamine Oxidase Assay Kit (QFDO-100) used for?
Answer: The Diamine Oxidase Assay Kit (QFDO-100) is designed for the quantification of diamine oxidase (DAO) activity in biological samples. It is particularly useful for studying histamine metabolism and gut health. Understanding DAO levels can provide insights into food intolerance issues and guide dietary adjustments. Researchers and clinical laboratories often utilize this kit to monitor histamine levels in patients, aiding in the diagnosis of various gastrointestinal conditions. -
Question:
How does the Diamine Oxidase Assay Kit work?
Answer: The Diamine Oxidase Assay Kit operates primarily through a colorimetric method that measures the enzymatic activity of DAO. The assay involves the conversion of histamine to imidazole acetaldehyde, which is then quantified through a color change. This method is advantageous because it allows for a direct assessment of DAO activity in your samples, providing reliable results essential for further research. It is especially beneficial in clinical diagnostics related to histamine intolerance and various digestive disorders. -
Question:
What samples can be tested with the QFDO-100 kit?
Answer: The QFDO-100 kit is versatile and can be used to test a variety of biological samples, including serum, plasma, and tissue extracts. Each type of sample may yield different results, so it's important to select the appropriate sample type based on your research needs. Laboratories often utilize this kit for analyzing human tissues or animal models in studies related to gut health and histamine-related conditions, providing valuable insights into DAO activity and its implications. -
Question:
Is the QFDO-100 kit suitable for research applications?
Answer: Yes, the Diamine Oxidase Assay Kit (QFDO-100) is highly suitable for various research applications, especially within the fields of biochemistry and medical research. Researchers use this kit to understand histamine metabolism and its impact on health. It can help in studying conditions like food intolerances or inflammatory bowel diseases, enriching scientific understanding and contributing to advancements in treatment options. Utilizing this kit allows researchers to gather essential data efficiently and accurately. -
Question:
What reagents are included in the QFDO-100 kit?
Answer: The Diamine Oxidase Assay Kit includes specific reagents essential for the assay, such as buffers, substrates, and the enzyme required for measuring DAO activity. Each reagent is formulated to ensure optimal conditions for accurate measurement of enzymatic activity. Detailed instructions are provided with the kit to help users prepare their samples and run the assays correctly. This ensures that researchers obtain reliable data for their studies into histamine metabolism and digestive health. -
Question:
Can the results from the QFDO-100 kit be used clinically?
Answer: Results obtained from the Diamine Oxidase Assay Kit (QFDO-100) can be valuable in clinical settings, as they help in diagnosing histamine intolerance and related gastrointestinal disorders. By measuring DAO activity, healthcare professionals can make informed decisions regarding diet and treatment options for patients. Furthermore, the kit can help track changes in DAO levels over time, offering insights into patient responses to specific treatments or dietary adjustments, enhancing patient care. -
Question:
How do I interpret the results from the QFDO-100 assay?
Answer: Interpreting the results from the Diamine Oxidase Assay involves comparing the optical density readings obtained from your samples against a standard curve created during the assay. This comparison allows you to quantify the DAO activity present. It’s important to understand the normal ranges for DAO levels to draw accurate conclusions, as abnormal levels may indicate digestive issues or histamine intolerance. Usually, researchers will contextualize results based on relevant literature to derive meaningful insights. -
Question:
What storage conditions are recommended for the QFDO-100 kit?
Answer: The Diamine Oxidase Assay Kit (QFDO-100) should be stored at 2-8 degrees Celsius to maintain reagent stability and integrity. Proper storage is crucial to ensure that the reagents remain effective for accurate assay results. It is also advisable to keep the kit away from direct sunlight and moisture, as these factors can adversely affect the components. Following these storage recommendations helps to maximize the shelf-life and reliability of your assay results. -
Question:
Are there any precautions to take when using the QFDO-100 kit?
Answer: When using the Diamine Oxidase Assay Kit, it is essential to follow the standard laboratory safety protocols, such as wearing appropriate personal protective equipment and handling all biological samples with care. Additionally, ensure that reagents are not contaminated during use and are properly aliquoted to avoid multiple freeze-thaw cycles that could denature enzymes or affect assay performance. These precautions help ensure accurate results and maintain laboratory safety. -
Question:
Where can I buy the Diamine Oxidase Assay Kit (QFDO-100) in Pakistan?
Answer: You can purchase the Diamine Oxidase Assay Kit (QFDO-100) from Ubuy in Pakistan. Ubuy offers a convenient online platform where you can find this kit along with various other laboratory products. Their user-friendly site allows you to easily navigate your options and place orders efficiently, ensuring you have everything you need for your research without hassle.
BioAssay Systems Assay Kits QFDO-100 Editorial Review
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PKR 219694
Order now and get it around Sunday, September 06
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Features & Benefits
- Non-radioactive, fluorimetric DAO assay for accurate histamine measurement.
- Detects diamine oxidase levels in serum or plasma with a sample size of just 10 μL.
- Achieves results in 30 minutes at room temperature without the need for a heater.
- Linear detection range from 0.5 to 6 U/L, ensuring precise quantification.
- Designed for high-throughput testing, compatible with 96-well plates.
- Ideal for diagnosing histamine intolerance effectively and efficiently.
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