What is Phosphate Buffered Saline (PBS) used for?

Phosphate Buffered Saline (PBS) is primarily used in biological research as a buffer solution for dissolving biological samples. Its formulation helps maintain a stable pH, making it ideal for applications like cell culture, immunoassays, and molecular biology experiments. The sterile nature of PBS prevents contamination, ensuring accurate results. For example, in cell culture, PBS is frequently used to rinse cells before trypsinization, as it helps maintain osmotic balance and cell viability.

Is PBS 1X suitable for use in cell culture?

Yes, PBS 1X is suitable for cell culture applications. It provides a balanced salt solution that supports the physiological conditions necessary for cell survival and growth. With its sterile formulation, PBS helps in processes such as washing cells, preparing samples for flow cytometry, and diluting other reagents. Many researchers use PBS to remove serum before enzymatic detachment of adherent cells, ensuring minimal disruption to experimental results.

How should Phosphate Buffered Saline be stored?

Phosphate Buffered Saline should be stored at room temperature in a cool, dry place, away from direct sunlight. It is recommended to keep PBS tightly sealed in its original container to prevent contamination. While PBS can be stored for extended periods, it is advisable to check for any visible contaminants before use. Proper storage ensures the buffer retains its efficacy, which is critical for sensitive applications such as immunofluorescence staining.

Can PBS be used in molecular biology techniques?

Yes, PBS is widely used in various molecular biology techniques, including PCR (Polymerase Chain Reaction), Western blotting, and ELISA (Enzyme-linked Immunosorbent Assay). Its role as a buffering agent aids in maintaining a stable pH during reactions, which is crucial for enzyme activity and specificity. For instance, during the dilution of antibodies or nucleic acids, PBS helps maintain the integrity of the reagents, contributing to reproducible experimental outcomes.

Is PBS 1X compatible with mammalian cells?

PBS 1X is compatible with mammalian cells, as it mimics physiological conditions, making it an ideal medium for cell handling and experiments. Its isotonic nature prevents osmotic shock, ensuring cell health during procedures like trypsinization or washing. Researchers often utilize PBS when preparing mammalian cells for flow cytometry assays, as it aids in maintaining cell viability while optimizing staining protocols.

What are the components of Phosphate Buffered Saline?

Phosphate Buffered Saline typically contains sodium chloride, potassium chloride, sodium phosphate, and potassium phosphate. These components work together to create an isotonic and buffered environment, maintaining a stable pH around 7.4. This balanced formulation is crucial for biological applications, as it prevents changes in cellular environments that could disrupt normal function. Understanding these components can help researchers select the right medium for their specific experiments.

Can I use PBS for preparing samples for flow cytometry?

Absolutely, PBS is a preferred buffer for preparing samples for flow cytometry. Its isotonic properties help preserve cell morphology and viability, which are essential for accurate analysis. By using PBS to dilute samples, researchers can effectively minimize background noise and improve the clarity of cytometric data. Additionally, many protocols recommend washing cells with PBS to reduce nonspecific binding of antibodies, enhancing the quality of results.

Is the PBS 1X solution sterile?

Yes, PBS 1X is available in a sterile formulation, ensuring it is free from microbial contamination. This sterility is critical in laboratory settings where contamination can compromise experimental integrity. For example, using sterile PBS is essential when handling cultured cells or preparing immunological assays to prevent bacterial or fungal interference. Always check that the packaging is intact to maintain the sterility of the solution before use.

Can I use PBS for washing tissue samples?

Yes, PBS is commonly used for washing tissue samples before further processing or analysis. Its saline composition helps maintain osmotic balance, preventing tissue distortion during washing. This is particularly important when preparing samples for histological examination or when conducting assays that require intact tissue morphology. Many researchers utilize PBS to wash samples thoroughly, ensuring accurate results in subsequent steps.

Where can I buy Phosphate Buffered Saline, PBS 1X, Sterile, 500 mL?

You can buy Phosphate Buffered Saline, PBS 1X, Sterile, 500 mL at Ubuy in Pakistan. Ubuy offers a wide selection of laboratory supplies, ensuring availability and convenience for researchers looking for high-quality PBS solutions. Shopping through Ubuy gives you access to reliable products tailored for scientific applications.

How to Shop Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL Online From Ubuy?

It’s easy to shop Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL Available to Shop Online in Pakistan?

Yes, at Ubuy Pakistan this product is available for you to shop at a reasonable price. The Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

eCon Lab Supply

Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL

1X Phosphate Buffered Saline (PBS) is a balanced, isotonic salt solution used for a variety of cell culture applications.

eCon Lab Supply

Item #: 51556604

Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL

Item #: 51556604

PKR 14991

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1X Phosphate Buffered Saline (PBS) is a balanced, isotonic salt solution used for a variety of cell culture applications.

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What Stands Out

Sterile Solution

Ensures contamination-free experiments, enhancing the reliability and accuracy of your research outcomes with ready-to-use phosphate buffered saline.

Convenient Volume

500 mL quantity provides ample supply for multiple applications, reducing the need for frequent reorders and saving laboratory time and resources.

Optimal pH Stability

Maintains a stable pH environment, crucial for biological experiments, ensuring consistent performance across various applications in cell culture and immunology.

Product Details

Manufactured in USA in ISO 9001 and 13485 certified facility
For research use only, not for human or animal clinical/therapeutic use
Each production lot undergoes stringent Good Manufacturing Practices and quality control tests
1X Phosphate Buffered Saline (PBS) is 0.22 micron filter-sterilized
Contains 137mM NaCl, 10mM NaH2PO4, 2.7mM KCl, 1.8mM KH2PO4 with pH 7.4
Used for cell culture applications such as washing cells, transporting cells, and preparing reagents

Container Type	Bottle
Item Form	Liquid
Item Weight	0.5 Pounds (230 grams)
Volume	500 Milliliters
Brand Name	eCon Lab Supply
Manufacturer	Ciro Mfg
Part Number	23.105.95
Included Components	Bottle
Brand	eCon Lab Supply
Item Volume	500 Milliliters
Package Information	Bottle

Who Should Buy?

Laboratory Research
Ideal for researchers conducting cell culture, protein purification, and immunoassays requiring a stable pH environment.
Diagnostic Testing
Useful for labs performing diagnostic tests, providing a sterile and buffered solution for sample preparation.
Biological Experiments
Suitable for biological experiments where maintaining osmotic balance and pH stability is critical for results.

Long-term Storage
Not suitable for long-term storage of cells or samples due to potential degradation and nutrient depletion.

Product Description

Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL

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Customer Questions & Answers

Question: How to Shop Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL Online From Ubuy?

Answer: It’s easy to shop Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL Available to Shop Online in Pakistan?

Answer: Yes, at Ubuy Pakistan this product is available for you to shop at a reasonable price. The Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

eCon Lab Supply Buffers Editorial Review

The phosphate-buffered saline solution is a high-quality product that is sterile and ready to use. It is a balanced salt solution that has a pH of 7.4, isotonicity with body fluids and is free of potential contaminants like endotoxins. The product is ideal for use in a range of laboratory applications that include cell culture, immunology and biochemistry. The PBS 1X solution can be used as a diluent, a buffer or as a washing solution, which makes it extremely versatile. Customer Feedback: Customers have been highly satisfied with this product, citing its convenience and ease of use as major advantages. They have found that the product is reliable and Consistent, which is essential for laboratory experiments. Additionally, the product has met expectations in terms of purity and sterility. Customers have also found that the product is competitively priced for the quality and quantity that it offers.

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Pros

High-quality, sterile product
Balanced salt solution with a pH of 7.4 and isotonicity
Versatile product that can be used in a range of laboratory applications
Diluent, buffer, or washing solution
Reliable and Consistent
Purity and sterility meet expectations
Competitive pricing

Product Price History

Important information

Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
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PKR 14991

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Features & Benefits

Contains a balanced, isotonic salt solution
Used to wash cells before dissociation
Transporting cells or tissue
Diluting cells for counting
Preparing numerous reagents
Manufactured under certified 14644 clean room conditions in USA lab

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Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL

PKR 14991

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What Stands Out

Product Details

Who Should Buy?

Product Description

Customer Questions & Answers

eCon Lab Supply Buffers Editorial Review

Customer Reviews & Ratings

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Pros

Product Price History

Important information

PKR 14991

Features & Benefits

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